Journal: bioRxiv

Article Title: XL-MS and De Novo Protein Design Identified a Common Motif for TREM2 Binding

doi: 10.64898/2026.04.23.720433

Figure Lengend Snippet: (a) SDS-PAGE analysis showing successful cross-linking of the heterodimeric TREM2 ECD /Trx-ApoE3 complex. (b) Representative high-quality MS/MS spectra of inter-protein cross-linked peptides. (c) Bar representation showing intra-protein XLs, inter-protein XLs, and inter-protein self-links. Figure was created using xiNET . ApoE3: light blue (N-terminal region, residues 1-167), light yellow (hinge region, residues 168-205), and pink (C-terminal region, residues 206-299).

Article Snippet: 20 μM Trx-ApoE3 (Sino Biological 10817-H30E) and 20 μM TREM2 ectodomain were incubated for 1 h at 4°C.

Techniques: SDS Page, Tandem Mass Spectroscopy

Journal: NeuroSci

Article Title: Effect of High-Salt Diet on Memory and Behavior in Mice Expressing Human Apolipoprotein Epsilon-4 (APOE4) Allele

doi: 10.3390/neurosci7020043

Figure Lengend Snippet: Animal demographics and experimental design. Male and female humanized APOE3 (strain 029018; n = 10 males and 10 females) and APOE4 (strain 027894; n = 10 males and 10 females) knock-in mice (5–7 months old) were initially maintained on a normal-salt diet (0.4% NaCl) to establish baseline metabolic and behavioral parameters (Week 2). Mice were then randomly assigned to either a low-salt (0.1% NaCl) or high-salt (4% NaCl) diet for 4 weeks, followed by metabolic and behavioral assessments (Week 8).

Article Snippet: Male and female APOE3 expressing (Strain 029018) and male and female APOE4 expressing (Strain 027894) mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA 04609).

Techniques: Knock-In

Journal: NeuroSci

Article Title: Effect of High-Salt Diet on Memory and Behavior in Mice Expressing Human Apolipoprotein Epsilon-4 (APOE4) Allele

doi: 10.3390/neurosci7020043

Figure Lengend Snippet: Effect of dietary salt on body parameters: body weight ( A ), food intake ( B ), water intake ( C ), and urinary volume ( D ) in APOE3 and APOE4 mice on normal salt (0.4% NaCl), low salt (0.1% NaCl) and high salt (4% NaCl). Each bar represents data as mean ± SE, n = 7 in low-salt treatment groups and n = 9 in high-salt treatment groups. Significance was calculated by Two-way ANOVA, followed by Bonferroni multiple comparison tests (* p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001).

Article Snippet: Male and female APOE3 expressing (Strain 029018) and male and female APOE4 expressing (Strain 027894) mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA 04609).

Techniques: Comparison

Journal: NeuroSci

Article Title: Effect of High-Salt Diet on Memory and Behavior in Mice Expressing Human Apolipoprotein Epsilon-4 (APOE4) Allele

doi: 10.3390/neurosci7020043

Figure Lengend Snippet: Memory and learning: the male and female APOE3 and APOE4 mice were trained, and their memory and learning abilities were determined by the Barnes maze test. The bars represent latency to target hole (sec) data as the mean ± SE on normal-salt (0.4%), low-salt (0.1% NaCl) ( A ) and high-salt (4% NaCl) ( B ) diets, n = 7 in the low-salt treatment groups and n = 9 in the high-salt treatment groups. No sex differences were observed. Significance was calculated by Two-way ANOVA, followed by Bonferroni multiple comparison tests (* p < 0.05).

Article Snippet: Male and female APOE3 expressing (Strain 029018) and male and female APOE4 expressing (Strain 027894) mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA 04609).

Techniques: Comparison

Journal: NeuroSci

Article Title: Effect of High-Salt Diet on Memory and Behavior in Mice Expressing Human Apolipoprotein Epsilon-4 (APOE4) Allele

doi: 10.3390/neurosci7020043

Figure Lengend Snippet: Memory in APOE3 and APOE4: 72 h after the 4th training day, memory to reach the target hole was measured by the Barnes maze test. Primary latency ( A , B ), primary errors ( C , D ), total nose pokes ( E , F ), and distance traveled ( G , H ) were measured. Each bar represents data as the mean ± SE, and n = 7 in the low-salt treatment groups and n = 9 in the high-salt treatment groups. No sex differences were observed. Significance was calculated by Two-way ANOVA, followed by Bonferroni multiple comparison tests. (* p < 0.05).

Article Snippet: Male and female APOE3 expressing (Strain 029018) and male and female APOE4 expressing (Strain 027894) mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA 04609).

Techniques: Comparison